In Vitro Human Display
Mabqi’s technology is based on proprietary fully human synthetic antibody libraries and highly innovative, flexible and robust display technology, especially fitted for challenging targets.
In Vitro Display: Our Technology of Choice
First result in a few weeks (ELISA, FACS...)
Direct access to antibody sequence
Fully human scaffold
Powerful in vitro selection
Peptides, proteins, whole cells
Depletion, epitope exlcusion, ...
No immune restriction
Access to epitopes "hindered" in mouse
* Mabqi library
In vitro display technologies, brewing for years in the academic environment, have recently been unleashed from patent restrictions allowing MAbqi to make its optimal use for the rapid generation of antibodies virtually against any target of any type (peptides, proteins, whole cells) and with high control over the antibodies selected.
Our Human Synthetic Libraries
The discovery of an antibody fragment that could retain its functionality inside cells and be produced stably in very high yields by Pierre Martineau and Sir Greg Winter (Nobel Prize in 2018) in Cambridge in 1998 opened up the doors to the generation of antibody fragment libraries and corresponding full antibodies with high developability properties. Over the years, these so called “naïve” or “single pot” antibody phage libraries derived from this unique clone have been used successfully by Inserm to derive highly specific and stable antibody reagents to a wide range of different target proteins, protein complexes, peptides and cells.
The latest libraries, HuscI2TM and FabIgTM (Inserm and iMAb joint ownership) combine the data gathered from the use of this scaffold, bioinformatics and structure modeling to optimize stability, paratope diversity, and affinity. Rational structure-based design and best-in-class precision technology have been used for accurate synthesis to maximize functionality and developability.
OUR SCAFFOLD: A KEY STRUCTURAL ELEMENT FOR HOMOGENEOUS HIGH EXPRESSION AND STABILITY
Our libraries are based on a single optimized scaffold reaching remarkably high levels of expression and stability.
Furthermore, the library diversity on this framework has been carefully designed to retain these properties resulting in collections of selected antibodies with homogeneous high expression and stability.
The human scaffold, diversification and hypervariable loop lengths variations are consistent with human antibodies.
Therefore all antibodies from our libraries, which reach a diversity of 3-10 billion, are humanized (-zumab) according to INN classification.
Besides the library, key to successful antibody recovery is clearly dependent on the process and screening protocols adopted to fit the projects specificities.
Over the years, we developed a robust toolbox for antigen preparation, a broad range of selection schemes including pH dependent antibody selections, scFv-Fab-mAb rapid reformating, screening, and numerous advanced bioanalytics to characterize and validate the Ab hits we provide for final lead selection. Our in-depth knowledge of phage display and scientific expertise in antigen preparation, assay development, and screening protocols, has resulted in successful delivery in all our projects.